Which Bacillus species is commonly used as a biological indicator for autoclave validation?

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Prepare for your Manor Preboards Module 6 Test with flashcards and multiple choice questions. Each question includes hints and explanations.

Multiple Choice

Which Bacillus species is commonly used as a biological indicator for autoclave validation?

Explanation:
Biological indicators for autoclave validation use spores that are very resistant to moist heat. Bacillus stearothermophilus is the standard choice because its spores withstand steam much more than the other Bacillus species listed, providing a stringent test of the cycle. If this indicator is inactivated, you can be confident the autoclave achieved the necessary conditions throughout the load; if it grows, the cycle didn’t meet requirements. The other species have spores that are not as reliably heat-resistant for steam validation, so they’re not as appropriate for this purpose.

Biological indicators for autoclave validation use spores that are very resistant to moist heat. Bacillus stearothermophilus is the standard choice because its spores withstand steam much more than the other Bacillus species listed, providing a stringent test of the cycle. If this indicator is inactivated, you can be confident the autoclave achieved the necessary conditions throughout the load; if it grows, the cycle didn’t meet requirements. The other species have spores that are not as reliably heat-resistant for steam validation, so they’re not as appropriate for this purpose.

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